CRITICAL ASPECTS OF MALE RAT FERTILITY IN THE ASSESSMENT OF EXPOSURE TO LAPROL-604
The present experiment studied the sexual behavior, biochemical profile of the testes, follicle stimulating hormone, luteinizing hormone and testosterone levels of male rats have been administered by Laprol-604 for 60 days. The surfactants are one of the potentially harmful chemicals released in the environment. The potentially toxic effects of surfactants are presently being studied with increasing intensity. Laprol-604 is a non-ionic surfactant which is widely used an ingredient of manufacturing epoxide rubber, enamels, varnishes, plastic, fiber, glues, emulsifiers, etс. Laprol-604 has been shown to produce some adverse health effects. A study was conducted to asses the effects of Laprol-604 on the reproductive system and sexual behavior of thirty sexually naive male Wistar rats. The Laprol-604 was administered to rats orally at dose levels of 1/10, 1/100 and 1/1000 LD50 – 12.5g/kg, respectively is the 1-st; 2-nd and 3-rd group for 60 days (one cycle of spermatogenesis). Sexual behavior was evaluated in a procedure consisting of an open field, it in the context of one interaction to one estrus female rat. Prolonged oral administration of Laprol-604 led to disturbance sn sexual behavior of male rats. Both the mount latency and intromission latency of male rat’s 1-st, 2-nd and 3-rd groups was significantly lengthened compared to controls. In contrast, the numbers of mountings and intromissions was significantly reduced in Laprol-604 treatment groups compared to control animals. Study has been found that control male rats could ejaculate 1.3 times/hour by contrast with male rats of 1-st, 2-nd and 3-rd groups which did not ejaculate. The significant relationships between the sexual activity and testosterone levels were found. In comparison to the control rats, there were the significant reductions in the weight of testes, epididymis, seminal vesicle and ventral prostate. Testicular and epididymal sperm density was decreased in the animals, which were treated with Laprol-604. Biochemical profile of the testis revealed a significant decline in the contents of protein, testicular cholesterol and glycogen. At the same time, the activity of testicular acid phosphatase was significantly increased, but decreasing alkaline phosphatase activity was found. The level of testosterone, luteinizing hormone and follicle stimulating hormone levels were significantly suppressed by Laprol-604. Results of this study clearly show that Laprol-604 induces the toxic impact on the male rats' reproductive system. The study found the relationship between the Laprol-604 administration effects on testosterone, luteinizing hormone and follicle stimulating hormone levels and sexual behavior of adult male rats.
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