• O.A. Polyvyana Poltava State Medical University, Poltava
  • V.I. Shepitko Poltava State Medical University, Poltava
  • Ye.V. Stetsuk Poltava State Medical University, Poltava
  • O.Ye. Akimov Poltava State Medical University, Poltava
  • O.S. Yakushko Poltava State Medical University, Poltava
  • O.V. Voloshyna Poltava State Medical University, Poltava
Keywords: liver, triptorelin embonate, oxidative stress, rat


In recent years, researchers have focused on the problem of the dependence of the functioning of various organs and systems on the level of androgens. The effect of long inhibition of testosterone synthesis by triptorelin on liver tissue is poorly understood. The aim of this research was to establish the microscopic organization of rat livers, production of nitric oxide and the intensity of oxidative stress in the rat livers during experimental central deprivation of luteinizing hormone synthesis by diphereline injection on the 270-360th day of the experiment. The experiments were carried out on 30 sexually mature male white rats of the Wistar line. Rats were divided into 2 groups: the control group (10) and the experimental group (20). Animals from the experimental group were subcutaneously injected triptorelin at a dose of 0.3 mg of the active substance/ per kg of body weight for 360 days, while the control group received an injection of saline. It was found that oxidative stress develops in hepatocytes, which is morphologically confirmed by karyopyknosis of the nuclei, oxyphilia of the cytoplasm with the appearance of a significant number of vacuoles in it. The vessels of the microcirculatory bed react with stasis. An increase in the production of superoxide radical anion in rat liver may be due to the absence of an inhibitory effect of testosterone on macrophages and liver mitochondria, which is accompanied by depletion of antioxidant enzymes and the development of oxidative stress. The intensity of biochemical markers of oxidative stress on the 360th day is lower than on the 270th day, which is due to an increase in the activity of antioxidant enzymes and a decrease in the production of reactive oxygen species.


Download data is not yet available.


1. Bahriy MM, Dibrova VA, Popadynets OH, Hryshchuk MI. Metodyky morfolohichnykh doslidzhen. Bahriy MM, Dibrova VA. redaktory. Vinnytsya: Nova knyha; 2016. 328s. [in Ukrainian]
2. Bell MA, Campbell JD, Joice G, Sopko NA, Burnett AL. Shifting the Paradigm of Testosterone Replacement Therapy in Prostate Cancer. World J Mens Health. 2018 May;36(2):103-109. doi: 10.5534/wjmh.170007.
2. Gur S, Alzweri L, Yilmaz-Oral D, Kaya-Sezginer E, Abdel-Mageed AB, Dick B, Sikka SC, Volkan Oztekin C, Hellstrom WJG. Testosterone positively regulates functional responses and nitric oxide expression in the isolated human corpus cavernosum. Andrology. 2020 Nov; 8(6):1824-1833. doi: 10.1111/andr.12866.
3. Kang J, Jia Z, Ping Y, Liu Z, Yan X, Xing G, Yan W. Testosterone alleviates mitochondrial ROS accumulation and mitochondria-mediated apoptosis in the gastric mucosa of orchiectomized rats. Arch Biochem Biophys. 2018 Jul 1;649:53-59. doi: 10.1016/
4. Komura K, Sweeney CJ, Inamoto T, Ibuki N, Azuma H, Kantoff PW. Current treatment strategies for advanced prostate cancer. Int J Urol. 2018 Mar;25(3):220-231. doi: 10.1111/iju.13512.
5. Merseburger AS, Hupe MC. An Update on Triptorelin: Current Thinking on Androgen Deprivation Therapy for Prostate Cancer. Adv Ther. 2016 Jul;33(7):1072-93. doi: 10.1007/s12325-016-0351-4.
6. Polyvyana O.A., Shepitko K.V., Stetsuk Ye.V., Akimov O.Ye., Dubinin D.S.. Influence of prolonged tripterelin-induced central deprivation of testosterone synthesis on morphological structure of rat’s liver. World of medicine and biology. 2021; 1(75): 205-209. DOI 10.26724/2079-8334-2021-1-75-205-209
7. Shim M, Bang WJ, Oh CY, Lee YS, Cho JS. Effectiveness of three different luteinizing hormone-releasing hormone agonists in the chemical castration of patients with prostate cancer: Goserelin versus triptorelin versus leuprolide. Investig Clin Urol. 2019 Jul;60(4):244-250. doi: 10.4111/icu.2019.60.4.244.
8. Son BK, Kojima T, Ogawa S, Akishita M. Testosterone inhibits aneurysm formation and vascular inflammation in male mice. J Endocrinol. 2019 Jun 1;241(3):307-317. doi: 10.1530/JOE-18-0646.
9. Son SW, Lee JS, Kim HG, Kim DW, Ahn YC, Son CG. Testosterone depletion increases the susceptibility of brain tissue to oxidative damage in a restraint stress mouse model. J Neurochem. 2016 Jan;136(1):106-17. doi: 10.1111/jnc.13371.
10. Stetsuk YeV, Akimov OYe, Shepitko KV, Goltsev AN. Structural organization of stromal and parenchymal components of rat testes during central deprivation of testosterone synthesis on the 180 day of the experiment. World of medicine and biology. 2020; 72(2): 203-207. Doi: 10.26724/2079-8334-2020-2-72-203-207.
How to Cite
Polyvyana, O., Shepitko, V., Stetsuk, Y., Akimov, O., Yakushko, O., & Voloshyna, O. (2021). MORPHOLOGICAL CHANGES AND OXIDATIVE HOMEOSTASIS IN THE LIVER TISSUES DURING LONG CENTRAL DEPRIVATION OF LUTEINIZING HORMONE SYNTHESIS BY TRIPTORELIN. The Medical and Ecological Problems, 25(5-6), 39-42.