THE REPRODUCTIVE INFLUENCE OF LAPROL-604 ON WISTAR RATS
This article introduces the results of research the Laprol-604-induced effects on the prenatal and early postnatal development of the Wistar rats. The high production volumes and widespread use of surfactants have been an environmental concern since the early 1960s. Surfactants are used in industry and agriculture and are also found in household and personal care products. The presence of surfactants and their biodegradation products in different environmental compartments can invoke a negative effect on the biota . The toxicity of surfactants to living organisms has been summarized in the scientific literature. Nevertheless, some information is still lacking in relation to the reproductive effects need to be understood in order to avoid unexpected adverse effects on future generations of people and the environment. Reproduction is a critical biological process of human and animal populations. It is known the embryo and fetus have immature blood–brain barrier, hepatic detoxifying, metabolizing properties and only rudimentary DNA repair mechanisms . Therefore, the embryo and fetus are more vulnerable than adults to the adverse effects of xenobiotics such as surfactant exposures. This study has been performed to increase the understanding of the adverse reproductive effects associated with Laprol-604 animals exposed during embryonic, fetal, and early postnatal development. Pregnant Wistar rats were administered 0,125; 1,25 and 12,5 mg/kg Laprol-604 once daily by gavage from the second gestation day (GD) to the twenty first GD. Controls consisted of 25 intact pregnant Wistar rats without Laprol-604 administration. Time of parturition for each animal, number of live pups and their conditions have been examined. All of live pups were daily counted, tabulated and they have been weighed several times during postnatal period. All live pups have been born by rats exposed to Laprol-604 12,5 mg/kg were pallid, inactive and died within the first 48 hours after birth. Approximately 50% of the pups of pregnant rats administrated 1,25 mg/kg died during the first 10 days after birth. Other 50% of these animals survived and reached puberty, but they had significant growth retardation. The surviving young rats had significantly higher liver weight compared to the control animals. Survival has been improved by lower dosage Laprol-604 administration and over 80% pups exposed to 0,125 mg/kg Laprol-604 have been born alive, stayed active for postnatal life and reached puberty. The results of study indicated that exposure of Laprol-604 to the pregnant rats caused decreasing postnatal survival of neonates, stunted growth in the surviving rat pups.
2. Babienko V., Sakharova I., Danilchenko L. Hygienic evaluation of the influence of the nitrogen-containing surfactants under conditions of acute experiment //Journal of the Grodno State Medical University. – 2015. –N. 4. – P. 85-87 [in Russian].
3. Bagmut I.Y. The influence of sub-toxic dose xenobiotic on immune response of experimental animals / I.Y. Bagmut, T.M. Popova, A.V. Titkova et al. // J of Education, Health and Sport. – 2016. –Vol. 6(11). – P. 629-639.
4. Calvin G, Long P () Ethylenediaminetetra (methylene phosphonic acid Genotoxity, biodistribution, subchronic and chronic toxicity in rats // Chemical toxicology. – 1988. – N. 26. – P. 601-610.
5. Council of Europe [France]. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg,18.III.1986, http://conventions.coe.int/treaty/en/ Treaties/Word/123.doc
6. EPA (US Environmental Protection Agency) (1991) Guidelines for Developmental Toxicity Risk Assessment. Risk Assessment forum, US Environmental Protection Agency, Washington/DC. EPA/600/FR-91-001
7. Grasty R. C. Effects of prenatal perfluorooctane sulfonate (PFOS) exposure on lung maturation in the perinatal rat / R.C. Grasty, J.A. Bjork, K.B. Wallace et al. // Birth Defect Res B. - 2005. – N. 74. – P. 405–416.
8. Grasty R.C. Prenatal window of susceptibility to perfluorooctane sulfonate-induced neonatal mortality in the Sprague-Dawley rat / R.C. Grasty, B.E. Grey, C.S. Lau et al. // Birth Defect Res B. – 2003. – N. 68. – P. 465–471.
9. Harada K. Historical and geographical aspects of the increasing perfluorooctanoate and perfluorooctane sulfonate contamination in human serum in Japan / K. Harada, A. Koizumi, N. Saito et al. // Chemosphere. – 2007. – N. 66. – P. 293–30.
10. Harpal S Buttar Embryotoxicity benzalkonium chloride in vaginally treated rats // Journal of Applied Toxicology. – 1985. – N. 5(6). – P. 398-401.
11. Hinderliter P.M. Perfluorooctanoate: placental and lactational transport pharmacokinetics in rats / Hinderliter P.M., Mylchreest E., Gannon S.A., et al. // Toxicology. – 2005. – N. 211. – P. 139 –148.
12. Ivanković T., Hrenović J. Surfactants in the environment //Arh. Hig. Rad. Toksikol. - 2010. – N.61(1). –P. - 95-110.
13. Knepper TP, Barcelo D and De Voogt P (2003) Analysis and Fate of Surfactants in the Aquatic Environment. Comprehensive analytical chemistry Volume XL ELSEVIER: 966
14. Lara-Martin P.A., Gomez-Parra A., and Gonzalez-Mazo E Sources, transport and reactivity of anionic and non-ionic surfactants in several aquatic ecosystems in SW Spain: a comparative study // Environmental Pollution. - 2008. – Vol.156 (1). – P. 36–45.
15. Lau C., Butenhoff J.L., Rogers J.M. The developmental toxicity of perfluoroalkyl acids and their derivatives. Toxicol Appl Pharmacol.n – 2004. – Vol. 198(2). – P. 231-4.
16. Murakami M. Occurrence and sources of perfluorinated surfactants in rivers in Japan / M. Murakami, E. Imamura, H. Shinohara, K. Kiri, Y. Muramatsu, A. Harada et al. // Environ Sci Technol. – 2008. – N. 42. – P. 6566–6572.
17. Newbold R., Heindel J. Developmental exposures and implications for early and latent disease. In: Environmental Impacts on Reproductive Health and Fertility Cambridge, UK Cambridge University Press. -2010. – P. 92–102.
18. Olkowska E., Polkowska Ż., Namieśnik J. Analytics of surfactants in the environment: problems and challenges // Chem. Rev. – 2011. – Vol. 111 (9). – P. 5667-5700.
19. Olkowska E., Ruman M., Kowalska A. and Polkowska Z. Determination of surfactants in environmental samples. Part III. Non-ionic compounds // Ecological Chemistry and Engineering. – 2013. - S 20 (3). – P. 449–461.
20. Petrovic M. Occurrence and distribution of nonionic surfactants, their degradation products, and linear alkylbenzene sulfonates in coastal waters and sediments in Spain / M. Petrovic, A. R. Fernández-Alba, F. Borrull et al. // Environmental Toxicology and Chemistry. – 2002. – Vol. 21(1). – P. 37–46.
21. Ramesh C Gupta (2011) Reproductive and Developmental Toxicology. Academic press ELSEVIER: 1223
22. RezunenkoYu. The effect of Laproxide L-303 on some hormonal exchange indices in prolonged toxification of white rats / Yu. Rezunenko, V . Zhukov, M. Kucheriavchenko et all. // J Clin Exp Med Res. – 2015. – Vol. 3(1). – P. 44-50 [in Russian].
23. Scherban N. G. Medical toxicological investigation of superficially active substances in connection with the problem of sanitary protection of drinking water sources / N.G. Scherban, V.I. Zhukov, V.A. Kapustnik et all. // International Medical Journal. – 2013. – N. 2. – P. 116-120 [in Russian].
24. World Health Organization and United Nations Environment Programme (2013) State of the Science of Endocrine Disrupting Chemicals–2012. Available: http://www.who.int/ceh/publications/endocrine/en/ [accessed 6 February 2014].
25. Ying G. G. Fate, behavior and effects of surfactants and their degradation products in the environment // Environ. Int. - 2006. – Vol.32 (3). –P. 417-431.
26. Yuan C. L. Study on characteristics and harm of surfactants / C. L. Yuan, Z. Z.Xu, M. X. Fan et al. // Journal of Chemical and Pharmaceutical Research. – 2014. – Vol. 6(7). – P. 2233-2237.
27. Zhukov V. Toxicologic and hygienic haracteristics of P-373-2-20; P-5003-AC; P-294-2-35 polyols an prognosis of their potential danger to environment / V. Zhukov, V. Telegin, O. Zaytseva et all // Science Research. - 2013. – Vol.1(2). – P. 31-34.
28. Zhukov V.I. (2000) Environmentally-hygienically description of superficially-active substances as contaminants of reservoirs. Kharkov Tornado:180 [in Russian].