SUSCEPTIBILITY OF PLANKTONIC AND FILM FORMS OF CANDIDA GLABRATA AND CANDIDA ALBICANS TO CATIONIC SURFACTANT ANTISEPTICS
The aim of the study was to investigate the sensitivity of planktonic and film forms of C. albicans and C. glabrata to cationic surfactant antiseptics. Materials and methods. The study was based on investigating 20 clinical strains of C. albicans and 15 C. glabrata isolated from surgical inpatientsю. The sensitivity of planktonic forms of investigated strains to antiseptic agents was quantitatively evaluated by two-fold serial dilutions (macrodilution) in Sabouraud liquid nutrient medium. Biofilm-forming properties of clinical strains C. albicans and C. glabrata were assessed by using the Christensen’s spectrophotometric method (MtP-test “microtiter plate test”). The influence of the antiseptics on C. albicans and C. glabrata film forms was assessed by the reproduction of the biofilms according to the above-described procedure with adding antiseptics in sub-bacteriostatic concentrations and the subsequent spectrophotometric ODU assessment. In the study we used antiseptics based on cationic surfactants, chlorhexidine digluconate 0.05 (Chlorhexidine-KR, manufactured by PJSC “Khimfarmzavod “Chervona zirka"”, Kharkiv, Ukraine (CHH)) and decamethoxin 0.2 (Decasan, produced by Yuria-Farm LLC ", Kyiv, Ukraine (DCM)). Results. According to the research results, lower sensitivity of C. glabrata strains to CHH was found, compared to the sensitivity of C. albicans strains. In addition, the activity of DCM in the investigated representatives of Candida spp. did not differ significantly. Clinical strains of C. glabrata were more susceptible to DCM compared to their susceptibility to CHH. C. albicans strains showed medium film-forming properties, while C. glabrata - high. The investigated cationic surfactant antiseptics possessed the same degree of activity on the film-forming properties of clinical strains of Candida spp. Conclusions. Cationic surfactant antiseptics (CHH and DCM) possess antifungal activity against planktonic and film forms of C. albicans and C. glabrata.
2. Wisplinghoff H, Bischoff T, Tallent SM, et al. Nosocomial bloodstream infections in US hospitals: Analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis. 2004 Aug 1;39(3):309-17.
3. Brown, G.D., Denning, D.W., Levitz, S.M. Tackling human fungal infections. Science, 2012,336:647.
4. World Health Organization. Global Guidelines for the Prevention of Surgical Site Infection [Internet]. Geneva, Switzerland: WHO. 2016. San Francisco: Matthew Holt. 2003 Oct [cited 2018 Aug 30]. Available from: http://apps.who.int/iris/bitstream/handle/10665/250680/9789241549882-eng.pdf;jsessionid=1F8A9546C46F1803027E22A3F82DBEE4?sequence=1
5. Enkler L, Richer D, Marchand AL, et al. Genome engineering in the yeast pathogen Candida glabrata using the CRISPR-Cas9 system. Sci Rep. 2016 Oct 21;6:35766.
6. Ananieva MM, Faustova MO, Loban GA’, AvetikovDS, et al. Microbiological aspects of chlorophyllipt extract used for prevention of candida postoperative complications. Euromediterranean Biomedical Journal. 2018;13(40):178-180.
7. Gabaldón T, Carreté L. The birth of a deadly yeast: tracing the evolutionary emergence of virulence traits in Candida glabrata. FEMS Yeast Res. 2016 Mar;16(2):fov110.
8. Brunke S., Hube B. Two unlike cousins: C andida albicans and C. glabrata infection strategies. Cellular microbiology. 2013;15(5):701-708.
9. Ananieva, M. M., M. O. Faustova, Ya O. Basarab. Antimicrobial effect of proteflazid extract on microflora of peri-implant areas in infectious and inflammatory complications after dental implantation. Zaporožskij Medicinskij Žurnal. 2017;6 (2017):809-812.
10. Nazarchuk, O., Faustova, M., Bobyr, V., & Kordon, Y. The investigation of the relationship between biofilm-forming properties of clinical strains of p.aeruginosa and their sensitivity to antiseptic medicines. Reports of Vinnytsia National Medical University. 2018;22(3):403-406.
11. Iraqui I, Garcia-Sanchez S, Aubert S, Dromer F, et al. The Yak1p kinase controls expression of adhesins and biofilm formation in Candida glabrata in a Sir4p‐dependent pathway. Mol Microbiol. 2005 Feb;55(4):1259-71.
12. Kumamoto, C.A. Candida biofilms. Curr Opin Microbiol. 2002;5:608–611.
13. Paliy G.K., Kovalchuk V.P., Fomina N.S. Paliy G. K. Characteristics of the modern arsenal of disinfectants and problems of disinfectology. Biomedical and biosocial anthropology. 2014;22:82–85.
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