L-ARGININE: GLYCINE-AMIDINOTRANSFERASE AS AN ALTERNATIVE BIOMARKER OF ANIMAL RENAL DAMAGE IN EXPERIMENTAL INTOXICATION

  • T.M. Popova Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine
Keywords: Laprol-604, surfactant, polyols, glomerular filtration rate, creatinine, urea, L-arginine: glycine amidinotransferase, kidneys

Abstract

Nowadays, surfactants are actively used in industry and household life. In this regard, the issue of studying their toxic effects on organs and the entire body is relevant. Early diagnosis of changes caused by xenobiotics is also necessary. Laprol-604 is one of numerous non-ionic surfactants, which due to its special physical chemical properties is widely used by the chemical-pharmaceutical industry. According to chemical properties, it is a complex mixture of organic polyoxypropylene polyols that are readily dissolved in water and organic solvents: acetone, and alcohols. This xenobiotic was selected due to the absence of data on reproductive effect in the scientific literature and the need to develop a prognosis of potential hazard to humans and warm-blooded animals. In this study, we strived to find out how laprol-604 influences on the functional state of adult rat kidneys. Three experimental groups of rats (10 male rats in each) were administered various doses of laprol-604 water solution: the first group was given 1/10 LD50; the second group - 1/100 LD50; the third group - 1/1000 LD50. The xenobiotic has been administered once daily by gavage at doses for 30 days. The control group consisted of 10 male rats on a standard ration of the vivarium without Laprol-604 administration. The laprol-604 toxic effect on the rat kidneys was estimated by determination of the concentration of total protein, creatinine, urea in serum and urine of rats. L-arginine: glycine amidinotransferase was additionally determined in the serum of rats. Administration of laprol-604 led to oliguria, hyperazotemia, uremia, and proteinuria as well as to the appearance of renal specific enzyme – L-arginine: glycine amidinotransferase in the serum of adult male rats. Therefore, the toxic impact of Laprol-604 on rats led to metabolic disturbances in animals, and the effect was dose-dependent.

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Published
2017-12-26
How to Cite
Popova, T. (2017). L-ARGININE: GLYCINE-AMIDINOTRANSFERASE AS AN ALTERNATIVE BIOMARKER OF ANIMAL RENAL DAMAGE IN EXPERIMENTAL INTOXICATION. The Medical and Ecological Problems, 21(5-6), 71-74. Retrieved from https://ecomed-journal.org/index.php/journal/article/view/109